A Review of Automated Microinjection of Zebrafish Embryos
نویسندگان
چکیده
منابع مشابه
A Fully Automated Robotic System for Microinjection of Zebrafish Embryos
As an important embodiment of biomanipulation, injection of foreign materials (e.g., DNA, RNAi, sperm, protein, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This paper presents a microrobotic system for fully automated zebrafish embryo injection, which overcomes the problems inherent in manual operati...
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One of the advantages of studying zebrafish is the ease and speed of manipulating protein levels in the embryo. Morpholinos, which are synthetic oligonucleotides with antisense complementarity to target RNAs, can be added to the embryo to reduce the expression of a particular gene product. Conversely, processed mRNA can be added to the embryo to increase levels of a gene product. The vehicle fo...
متن کاملGlass-capillary-accessible Dynamic Microarray for Microinjection of Zebrafish Embryos
This paper describes a glass-capillary-accessible dynamic microarray for an efficient microinjection of zebrafish embryos. The microfluidic channel has an array of holes at the trapping sites and enabled a rapid alignment of eggs, direct injection, observation, and selective retrieval of embryos. Fertilized zebrafish eggs can be loaded without pumping, and genes of interest or antisence oligos ...
متن کاملMicroinjection into zebrafish embryos (Danio rerio) - a useful tool in aquatic toxicity testing?
Background: Microinjection was tested as a potentially powerful tool to introduce natural and anthropogenic pollutants directly into fish eggs to determine their toxicological impact on fish. With this technique, parental transfer of lipophilic contaminants may be mimicked. Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) eggs with sp...
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ژورنال
عنوان ژورنال: Micromachines
سال: 2018
ISSN: 2072-666X
DOI: 10.3390/mi10010007